Benzoylation

This procedure is used in the PAS reaction to block hydroxyl and amino groups. It does this by forming esters with them. As a consequence some carbohydrates fail to react with the Schiff’s reagent. This may be used as a means of identifying them, particularly if done in conjunction with other blocking procedures.

Solution

Procedure

1. Place dewaxed sections into the solution at the temperature specified for the time given.