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Acridine Orange

Acridine Orange

Class: Acridine
Ionisation: Basic

Chemical structure of Acridine Orange

Common Name: Acridine orange
Suggested Name: Acridine orange
Other Names:

C.I. Number: 46005
C.I. Name: Basic orange 14
Color: Yellow
Solubility Aqueous: Readily
Solubility Ethanol: Readily
Absorption Maximum: 497, 467 (Conn)489 (Aldrich)539 (Gurr)
Empirical Formula: C17H20N3Cl
Formula Weight: 301.8


Acridine orange has been recommended as a fluorochrome to differentiate between DNA (green fluorescence) and RNA (red fluorescence). It also makes a good fluorescent Schiff reagent.


  1. R. D. Lillie.
    Conn’s Biological Stains
    Originally published by: The Blakiston Co.
    Williams & Wilkins, Baltimore, MD., U.S.A.
  2. Edward Gurr, (1971)
    Synthetic dyes in biology, medicine and chemistry
    Academic Press, London, England.
  3. Aldrich Chemical Catalogue, 1992
    Aldrich Chemical Company, Milwaukee, WI, USA.