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Auramine O

Auramine O

Ionisation: Basic

Chemical structure of Auramine O

Common Name: Auramine O
Suggested Name: Auramine O
Other Names:

C.I. Number: 41000
C.I. Name: Basic yellow 2
Color: Yellow
Solubility Aqueous: 0.74%
Solubility Ethanol: 0.74%
Absorption Maximum: 432, 380 (Conn) 434, 370 (Aldrich) 430 (Gurr)
Empirical Formula: C17H22N3Cl
Formula Weight: 321.9


Auramine O is used as a fluorescent stain for demonstrating acid fast organisms in a method similar to the Ziehl Neelsen. It also can be used to make a fluorescent Schiff's reagent.


  1. R. D. Lillie.
    Conn’s Biological Stains
    Williams & Wilkins, Baltimore, MD., U.S.A.
  2. Aldrich chemical catalogue, 1992
    Aldrich Chemical Company, Milwaukee, WI, USA.
  3. Edward Gurr, 1971
    Synthetic dyes in biology, medicine and chemistry
    Academic Press, London, England.