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Iodonitrotetrazolium

Dye

Iodonitrotetrazolium

Class: Tetrazolium salt
Ionisation: Basic

Chemical structure of Iodonitrotetrazolium

Common Name: INT
Suggested Name: Iodonitrotetrazolium
Other Names:

–

C.I. Number: –
C.I. Name: –
Color: Red
Solubility Aqueous: Soluble
Solubility Ethanol: Soluble
Absorption Maximum: –
Empirical Formula: C19H13N5O2ICl
Formula Weight: 505.7

Description

Iodonitrotetrazolium chloride has been used to visualize dehydrogenase enzyme activity histochemically. As the enzyme removes hydrogen from the substrate it is transferred to a hydrogen acceptor, usually a co-enzyme. From the co-enzyme, it is transferred to the tetrazole, which is consequently reduced to its insoluble and intensely colored formazan. The formazan of INT is red.

References

  1. R. D. Lillie.
    Conn’s Biological Stains
    Williams & Wilkins, Baltimore, MD., U.S.A.
  2. Aldrich chemical catalogue, 1992
    Aldrich Chemical Company, Milwaukee, WI, USA.
  3. Burstone, M.S.
    Enzyme Histochemistry and its application to the study of neoplasms
    Academic Press, New York, NY, USA