Swiss blue
Description
Methylene blue is a very commonly used dye. It stains nuclei blue and is often used for that purpose. Following "ripening", a process involving atmospheric, or chemical, oxidation and the production of the three strongly metachromatic dyes Azure A, B, and C. This solution is called polychrome methylene blue and is very valuable for staining rapid frozen sections. Due to its strong metachromatic staining properties, it can be used for the demonstration of mucins, cartilage, mast cells, etc. A comparison of these dyes shows the relationship clearly.
References
- R. D. Lillie.
Conn’s Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A. - Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA. - Edward Gurr, 1971
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England. - Susan Budavari, Editor,
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA