Chamberlain’s Fluid
Formula
Material | Amount | |
---|---|---|
Chromium trioxide | 1 | g |
Acetic acid, glacial | 2 | mL |
Water | 90 | mL |
Osmium tetroxide, 1% aqueous | 8 | mL |
Description
Dissolve the chromium trioxide in the water, then add the acetic acid. Just before use add the osmium tetroxide.
This is a modification of Flemming’s strong fluid. It is used for cytological preservation, chromosomes particularly. As with all of these osmium tetroxide containing fluids, the tissue should be 2 mm or so thick, no more.
Time
Pieces less than 2 millimeters should be fixed within 24 hours.
Aftertreatment
Wash in running tap water overnight.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Davenport, H.A., (1960),
Histological and histochemical techniques,
W. B. Saunders Co., London, UK., Oxford, UK.