Methacarn
Formula
| Material | Amount | |
|---|---|---|
| Methanol, absolute | 60 | mL |
| Acetic acid, glacial | 10 | mL |
| Chloroform | 30 | mL |
Description
Methacarn is Carnoy’s fluid in which methanol replaces the ethanol. This causes less shrinkage of the tissue so that morphological preservation is superior to Carnoy. It also preserves antigenic sites more effectively than does Carnoy or other ethanol fixatives. It has been recommended for studies with DNA and RNA.
Time
4 hours or longer.
Aftertreatment
Transfer to absolute ethanol.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Puchtler, H., Sweat Waldrop, F., Meloan, S.N., Terry, M.S. and Conner, H.M. (1970)
Methacarn (methanol-Carnoy) fixation
Journal of Histochemistry and Cell Biology, v. 21, N° 2., June 1970.
