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Zinc Formalin

Zinc Formalin


Strong formalin100mL
Zinc chloride1.6g
Sodium chloride4.5g
Distilled water900mL

Zinc sulphate or salicylate may be used instead of zinc chloride. The pH may be adjusted with 1M HCl or NaOH.


Fixation of average sized pieces should be satisfactory after 24 hours, but may be left for several days. Wash tissues with running tap water for half an hour or so before processing.

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.


  1. Kiernan, J.A.,
    Histological & Histochemical Methods, Ed. 3,
    Butterworth Heinemann, Oxford, UK.