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Celestine Blue Hemalum An Acid Resistant Nuclear Stain

Protocol

Celestine Blue Hemalum

An Acid Resistant Nuclear Stain

6
steps
2
materials
print

Materials

Tissue Sample

5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.


Protocol

  1. Bring sections to water via xylene and ethanol.
  2. Place into the iron alum-celestine blue solution for 10 minutes.
  3. Wash with tap water.
  4. Place into the alum hematoxylin for 10 minutes.
  5. Wash with water to blue.
  6. Continue with the counterstain

Expected Results

  • Nuclei – blue-black
  • Other tissue – according to the counterstain

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.


References

  1. Culling, C.F.A., Allison, R.T. and Barr, W.T.
    Cellular Pathology Technique, Ed.4.
    Butterworth, London, UK.
  2. Bancroft, J.D. and Stevens A. (1982)
    Theory and practice of histological techniques Ed. 2
    Churchill Livingstone, Edinburgh & London, UK.
  3. Humason, G. L., (1967)
    Animal tissue techniques, Ed. 2
    W. H. Freeman and Company, San Francisco, Ca, USA
  4. Drury, R.A.B. and Wallington, E.A., (1980)
    Carleton’s histological technique Ed. 5
    Oxford University Press, Oxford, UK.
  5. Putt, F. A.
    Manual of Histopathological Staining Methods
    John Wiley & Sons, New York, NY., USA