Crétin’s Iron Hematoxylin

Crétin's Iron Hematoxylin

8

steps

6

materials

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Materials

Solution A

Material	Amount	Function
Ferrous sulfate	4 g	Mordant
Distilled water	100 mL	Solvent

Solution B

Material	Amount	Function
Potassium ferrocyanide	2 g	Mordant
Potassium ferricyanide	1 g	Mordant
Distilled water	100 mL	Solvent

Solution C

Material	Amount	Function
Hematoxylin	0.5 g	Dye
Distilled water	100 mL	Solvent

Solution D

Material	Amount	Function
Ferric ammonium sulfate	5 g	Mordant
Distilled water	100 mL	Solvent

Compounding procedure

Make each solution seperately.

Protocol

Bring sections to water with xylene and ethanol.
Place into solution A for 24 hours.
Wash with running tap water overnight.
Place into solution B for 3-6 hours.
Rinse with distilled water.
Place into solution C overnight.
Place into solution D until differentiated.
Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected Results

Nuclei – dense black

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.

References

Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.