Heidenhain’s Vanadium Hematoxylin
Materials
Stock A
| Material | Amount | Function |
|---|---|---|
| Hematoxylin | 0.5 g | Dye |
| Distilled water | 100 mL | Solvent |
Stock B
| Material | Amount | Function |
|---|---|---|
| Ammonium vanadate | 0.25 g | Mordant |
| Distilled water | 100 mL | Solvent |
Working Solution
| Material | Amount | Function |
|---|---|---|
| Stock A | 60 mL | Dye |
| Stock B | 30 mL | Mordant |
Compounding Procedure
- Make each stock solution separately.
- Combine in the specified proportions.
Protocol
- Bring sections to water with xylene and ethanol.
- Place into the staining solution for an appropriate time.
- Rinse well with water.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Expected Results
- No details were given.
Notes
- The staining time should be determined by trial.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
Citing:
Böhm and Oppel, (1907)
Manuel de technique microsopique, ed. 4, p. 105.
Vigot, Paris, France
