Protocol
Hitchcock Ehrich
for Plasma Cells
This method uses malachite green and acridine red to stain plasma cells in a similar manner to the methyl green pyronin methods.
Materials
Solution A
| Material | Amount | |
|---|---|---|
| Malachite green | 1 | g |
| Distilled water | 100 | mL |
Solution B
| Material | Amount | |
|---|---|---|
| Acridine red | 3 | g |
| Distilled water | 100 | mL |
Combine 1 part of solution A with 3 parts of solution B immediately before use.
Tissue Sample
Zenker fixation is recommended. Other fixatives may not be satisfactory.
Protocol
- Bring sections to water via xylene and ethanol.
- Place into the staining solution for 30 seconds.
- Rinse with water.
- Dehydrate rapidly with absolute ethanol.
- Clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – green
- Plasma cell cytoplasm – crimson
- Other cell cytoplasm – pink
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Biological Staining Methods, 6th ed. (1957)
Gurr George T.,
George T. Gurr, London, UK
