Skip to main content

Klatskin’s Modification of Masson’s Trichrome for Liver

Klatskin's Modification of Masson's Trichrome

for Liver



  • Harris’ alum hematoxylin.
  • Solution A
    Picric acidto saturation
    Ethanol, 95%100mL
  • Solution B
    Xylidine ponceau1g
    Distilled water99mL
    Glacial acetic acid1mL
  • Solution C
    Phosphomolybdic acid1g
    Distilled water100mL
  • Solution D
    Aniline blueto saturation
    Glacial acetic acid2.5mL
    Distilled water97.5mL
  • Solution E
    Acetic acid, glacial1mL
    Distilled water99mL

Tissue Sample

3-5 micron paraffin sections of formalin fixed liver.


  1. Bring sections to water via xylene and ethanol
  2. Stain with Harris’ hemalum for 10 minutes.
  3. Rinse with 95% ethanol.
  4. Place in solution A for 10 minutes.
  5. Wash with water for 10 minutes.
  6. Place in solution B for 5 minutes.
  7. Place in solution C for 5 minutes.
  8. Place in solution D for 2 minutes.
  9. Replace in solution C for 5 minutes.
  10. Place in solution E for 5 minutes.
  11. Place in 70% ethanol for 2 minutes.
  12. Dehydrate with ethanol, clear and mount with a resinous medium.

Expected Results

  • Cytoplasm  –  red
  • Erythrocytes  –  red
  • Muscle  –  red
  • Collagen  –  blue
  • Nuclei  –   brown


  • Aniline blue, is a mixture of two dyes, methyl blue, and water blue. Either may usually be substituted satisfactorily.
  • Although recommended for liver sections, this modification may also be used as a general replaceement for Masson’s method.

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.


  1. Petersen, K.F., West, A.B., Reuben, A., Rothman, D. and Shulman, G.I. (1996)
    Noninvasive Assessment of Hepatic Triglyceride Content in Humans With 13C Nuclear Magnetic Resonance Spectroscopy.
    Hepatology, V. 24, p 114-117