Skip to main content
search

Yasvoyn’s Iron Hematoxylin

Protocol

Yasvoyn's Iron Hematoxylin

5
steps
3
materials
print

Materials

Solution A

MaterialAmountFunction
Hematoxylin0.1 gDye
Distilled watermLSolvent

Solution B

MaterialAmountFunction
Ferric ammonium sulfate2.5 gMordant
Distilled water100 mLSolvent

Compounding Procedure

  1. Make each solution separately.
  2. For use, add solution B drop by drop to 20 drops of solution A until it just remains blue .
  3. The working solution may be used immediately, but is not stable for long.

Protocol

  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for 2-5 minutes.
  3. Rinse with water.
  4. Remove excess stain with 70% ethanol if necessary.
  5. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected Results

  • Nuclei  –  black
  • Background  –  as counterstain or unstained

Notes

  • The stock solutions are stable for some time.
  • The working solution should be made fresh.
  • Although not specified, a tap water wash to blue the hematoxylin may be useful following step 4.
  • Following a wash to blue the nuclei, a counterstain could probably be applied, if wished, before dehydration.

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.

References

  1. Gray, Peter. (1954)
    The Microtomist’s Formulary and Guide.
    Originally published by: The Blakiston Co.
    Republished by: Robert E. Krieger Publishing Co.
    Citing:
    Roskin, G.E, (1946).
    Mikroskopecheskaya technica, p. 150