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Coating Tissue Sections with Celloidin

How to Coat Tissue Sections with Celloidin

This procedure is used when a treatment is to be applied that may remove the sections from the slide, usually a strong alkali, or when there is a strong possibility of a coarse precipitate forming that may settle on the tissue.


Absolute ethanol50mL
Note: Ether is explosive and should be handled with care. Ensure there are no open flames in the vicinity.


  1. Ensure sections are in absolute ethanol.
  2. Place into the celloidin solution for one minute
  3. Stand on end on absorbent paper for about a minute to drain the excess, but do not allow to dry.
  4. Place into 80% ethanol for 5 minutes to harden.
  5. Continue with the procedure.
  6. Before clearing and coverslipping, the celloidin may be removed with an equal parts mixture of ether and absolute ethanol or with agitation in acetone. However, the treatment may have made the tissue fragile and it may float off the slide. In some cases, it may be preferable to coverslip with the celloidin layer intact.

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.


  1. Lillie, R.D., (1954)
    Histopathologic technique and practical histochemistry Ed.2
    Blakiston, New York, USA