The chart below compares the ratio of mordant to dye in aluminum hematoxylin formulae. Weights are in grams, ratios are number:1. Immediately following the chart is the information on which the calculations were based. Slight discrepancies in the figures are due to rounding.
Note: Ehrlich’s formula specifies saturation of the solvent with potassium alum. The solvent is an equal parts mixture of water, glycerol and ethanol with a small amount of acetic acid. Potassium alum saturates in water at just under 14% and is freely soluble in glycerol, but the amount the solvent mixture dissolves is not given. The amount of potassium alum listed for Ehrlich’s formula is an estimate and is not based on any information
| Formulation | Dye to Mordant Ratio | Dye Amount (g) | Mordant (Aluminum Source) | Aluminum | Ratio N:A | ||
|---|---|---|---|---|---|---|---|
| Source | Amount (g) | Amount Need for 1:1 Dye:Mordant Ratio (g) | Amount Available in Mordant (g) | ||||
| Anderson 1923a | 8 | 2.5 | Ammonium Alum | 20 | 0.22 | 1.19 | 5.3 |
| Anderson 1923b | 50 | 2.5 | Ammonium Alum | 125 | 0.22 | 7.44 | 33.1 |
| Anderson 1929 | 6 | 5 | Ammonium Alum | 30 | 0.45 | 1.79 | 4 |
| Apathy | 10 | 3 | Ammonium Alum | 30 | 0.27 | 1.79 | 6.6 |
| Baker Hematal-8 | 8.4 | 0.94 | Aluminum Sulphate | 8 | 0.08 | 1.24 | 14.7 |
| Baker Hematal-16 | 16.8 | 0.47 | Aluminum Sulphate | 8 | 0.04 | 1.24 | 29.4 |
| Bencosme | 67.5 | 2 | Potassium Alum | 133 | 0.18 | 7.57 | 42.1 |
| Bennett | 90 | 1 | Potassium Alum | 90 | 0.09 | 5.12 | 57 |
| Bosma | 10 | 2.5 | Ammonium Alum | 25 | 0.22 | 1.49 | 6.6 |
| Bullard | 2.5 | 8 | Ammonium Alum | 60 | 0.72 | 3.57 | 4.95 |
| Carazzi | 50 | 1 | Potassium Alum | 50 | 0.09 | 2.84 | 31.6 |
| Cole 1903 | 1 | 6 | Ammonium Alum | 6 | 0.54 | 0.36 | 0.7 |
| Cole 1943 | 66.7 | 1.5 | Ammonium Alum | 100 | 0.13 | 5.95 | 44.2 |
| Debiden | 20 | 5 | Potassium Alum | 100 | 0.45 | 5.69 | 12.7 |
| de Groot | 11 | 2 | Ammonium Alum | 22 | 0.18 | 1.31 | 7.3 |
| Delafield | 15 | 6 | Ammonium Alum | 90 | 0.54 | 5.36 | 9.9 |
| Ehrlich | 8.3 | 6 | Potassium Alum | 50 | 0.54 | 2.84 | 5.3 |
| Friedlander | 1 | 6 | Potassium Alum | 6 | 0.54 | 0.34 | 0.6 |
| Gadsdon | 10.9 | 5.5 | Potassium Alum | 60 | 0.49 | 3.41 | 6.9 |
| Gage | 40 | 1 | Potassium Alum | 40 | 0.09 | 2.28 | 25.3 |
| Galigher | 0.6 | 5 | Ammonium Alum | 3 | 0.45 | 0.18 | 0.4 |
| Garvey | 18 | 2.5 | Potassium Alum | 45 | 0.22 | 2.56 | 11.4 |
| Gill I | 8.8 | 2 | Aluminum Sulphate | 18 | 0.18 | 2.78 | 15.4 |
| Gill III | 17.6 | 4 | Aluminum Sulphate | 70 | 0.36 | 11.1 | 30.9 |
| Gill III | 26.4 | 6 | Aluminum Sulphate | 158 | 0.54 | 24.98 | 46.3 |
| Graham | 12.5 | 2 | Aluminum Sulphate | 25 | 0.18 | 3.94 | 21.9 |
| Hamilton | 37.5 | 2 | Ammonium Alum | 75 | 0.18 | 4.46 | 24.8 |
| Harris | 20 | 5 | Potassium Alum | 100 | 0.45 | 5.69 | 12.6 |
| Harris & Power | 3 | 20 | Potassium Alum | 60 | 1.8 | 3.41 | 1.9 |
| Haug | 0.9 | 5.5 | Aluminum acetate | 5 | 0.49 | 0.96 | 1.9 |
| Hine | 3.8 | 1 | Aluminum Sulphate | 4 | 0.09 | 0.59 | 6.6 |
| Horneyold | 0.5 | 8.4 | Ammonium Alum | 4 | 0.75 | 0.25 | 0.3 |
| Krutsay | 50 | 1 | Potassium Alum | 50 | 0.09 | 2.84 | 31.6 |
| Langeron 1924 | 12.5 | 4 | Ammonium Alum | 50 | 0.36 | 2.84 | 7.9 |
| Langeron 1942 | 50 | 1 | Potassium Alum | 50 | 0.09 | 2.84 | 31.6 |
| Launoy | 0.5 | 10 | Potassium Alum | 5 | 0.9 | 0.79 | 0.9 |
| Lee | 50 | 1 | Ammonium Alum | 50 | 0.09 | 2.98 | 33.1 |
| Lillie | 10 | 5 | Ammonium Alum | 50 | 0.45 | 2.98 | 6.6 |
| McLachlan var I | 8.8 | 2 | Aluminum Sulphate | 17.5 | 0.18 | 2.76 | 15.4 |
| McLachlan var II | 12.5 | 2 | Ammonium Alum | 25 | 0.18 | 1.49 | 8.3 |
| Mallory | 20 | 2.5 | Potassium Alum | 50 | 0.22 | 2.84 | 12.7 |
| Mallory & Wright | 140 | 1 | Ammonium Alum | 140 | 0.09 | 8.33 | 92.7 |
| Mann | 5.8 | 6 | Potassium Alum | 35 | 0.54 | 1.99 | 3.7 |
| Martinotti | 7.5 | 2 | Potassium Alum | 15 | 0.18 | 0.89 | 5 |
| Masson | 3 | 20 | Potassium Alum | 60 | 1.8 | 3.41 | 1.9 |
| Mayer 1891, 1903 | 50 | 1 | Ammonium Alum | 50 | 0.09 | 2.98 | 33.1 |
| Mayer 1901 | 50 | 1 | Potassium Alum | 50 | 0.09 | 2.84 | 31.6 |
| Mayer 1896 | 12.5 | 4 | Ammonium Alum | 50 | 0.36 | 2.98 | 8.3 |
| Molnar I | 5 | 10 | Ammonium or potassium alum | 50 | 0.9 | 2.98 or 2.85 | 3.3 or 3.2 |
| Molnar II | 12.5 | 4 | Ammonium or potassium alum | 50 | 0.36 | 2.98 or 2.85 | 8.3 or 7.9 |
| Papamiltiades | 2.5 | 4 | Aluminum Sulphate | 10 | 0.36 | 1.58 | 4.4 |
| Pusey | 37.5 | 1.6 | Ammonium Alum | 60 | 0.14 | 3.57 | 24.8 |
| Rawitz 1895a | 1 | 10 | Potassium Alum | 10 | 0.9 | 0.57 | 0.6 |
| Rawitz 1895b | 6 | 2.5 | Ammonium Alum | 15 | 0.22 | 0.89 | 4 |
| Rawitz 1909 | 10 | 2 | Aluminum nitrate | 20 | 0.18 | 1.44 | 8 |
| Reddy | 9.4 | 6.4 | Ammonium Alum | 60 | 0.58 | 3.57 | 6.2 |
| Sass var I | 14 | 10 | Ammonium Alum | 140 | 0.9 | 8.33 | 9.3 |
| Sass var II | 50 | 1 | Ammonium Alum | 50 | 0.09 | 2.98 | 33.1 |
| Schmorl | 20 | 5 | Ammonium Alum | 100 | 0.45 | 5.95 | 13.2 |
| Scott | 7 | 3 | Potassium Alum | 21 | 0.27 | 1.19 | 4.4 |
| Slidders | 100 | 1 | Potassium Alum | 100 | 0.09 | 5.69 | 63.3 |
| Unna | 46.7 | 3 | Ammonium Alum | 140 | 0.27 | 8.33 | 30.9 |
| Watson | 1 | 6 | Ammonium Alum | 6 | 0.54 | 0.36 | 0.7 |
