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Pyronin Y

Pyronin Y

Class: Pyronin
Ionisation: Basic

Chemical structure of Pyronin Y

Common Name: Pyronin Y
Suggested Name: Pyronin Y
Other Names:

Pyronin G

C.I. Number: 45005
C.I. Name:
Color: Red
Solubility Aqueous: 8.96%
Solubility Ethanol: 8.96%
Absorption Maximum: 545 (Gurr) 548 (Aldrich) 552 (Conn)
Empirical Formula: C17H19N2OCl
Formula Weight: 302.8


Pyronin Y is used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna-Pappenheim method.

Compare the formula with other pyronins.


  1. R. D. Lillie.
    Conn’s Biological Stains
    Williams & Wilkins, Baltimore, MD., U.S.A.
  2. Aldrich chemical catalogue, 1992
    Aldrich Chemical Company, Milwaukee, WI, USA.
  3. Susan Budavari, Editor,
    The Merck Index, Ed. 12
    Merck & Co., Inc., Whitehouse Station, NJ, USA
  4. Edward Gurr, 1971
    Synthetic dyes in biology, medicine and chemistry
    Academic Press, London, England.