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Sudan Black B

Sudan Black B

Class: Azo
Ionisation: Lysochrome

Chemical structure of Sudan Black B

Common Name: Sudan black B
Suggested Name: Sudan black B
Other Names:

Fat black HB

C.I. Number: 26150
C.I. Name: Solvent black 3
Color: Blue-black
Solubility Aqueous: Insoluble
Solubility Ethanol: Insoluble
Absorption Maximum: 596-605 (Conn) 598, 415 (Aldrich)
Empirical Formula: C29H24N6
Formula Weight: 456.6


Sudan black B is a lysochrome (fat soluble dye) predominantly used for demonstrating triglycerides in frozen sections. It is also valuable for demonstrating some protein bound lipids in paraffin sections. It may also stain other materials, not being completely restricted to lipids as the other dyes used.

Compare this dye to the other common lysochromes sudan III, sudan IV and oil red O.


  1. R. D. Lillie.
    Conn’s Biological Stains
    Williams & Wilkins, Baltimore, MD., U.S.A.
  2. Aldrich chemical catalogue, 1992
    Aldrich Chemical Company, Milwaukee, WI, USA.
  3. Edward Gurr, 1971
    Synthetic dyes in biology, medicine and chemistry
    Academic Press, London, England.