De-acetylation
For the Periodic Acid-Schiff Reaction
This procedure is also called saponification. It reverses the effects of acetylation and restores Periodic Acid-Schiff positivity. However, this is not absolute, and proceeds at different rates for different compounds. It may therefore be used to identify compounds based on double criteria:
- Blocking of staining by acetylation, and
- Restoration of staining by deacetylation
Solution
| Material | Amount | |
|---|---|---|
| Ethanol, absolute | 30 | mL |
| Distilled water | 10 | mL |
| Ammonia water, 28% | 10 | mL |
Procedure
- Celloidinize previously acetylated sections.
- Place into the solution in a sealed container at 37°C for 4-24 hours.
Tissue Temperature Time State Glycogen 37°C 4-6 hours Restored Intestinal mucins 37°C 4-6 hours Restored Basement membrane 37°C 16 hours Restored Brush borders 37°C 16 hours Restored Retinal rods 37°C 24 hours Restored - Rinse well with 80% ethanol.
- Continue on with the staining method.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA
