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Amido Black 10B

Amido Black 10B

Class: Azo
Ionisation: Acid

Chemical structure of Amido Black 10B

Common Name: Amido black 10B
Suggested Name: Amido black 10B
Other Names:

Amidoschwarz Naphthol blue black

C.I. Number: 20470
C.I. Name: Acid black 1
Color: Blue black
Solubility Aqueous: Readily
Solubility Ethanol: Readily
Absorption Maximum: 618 (Aldrich) 624 (Gurr)
Empirical Formula: C22H14N6O9S2Na2
Formula Weight: 616.516


In conjunction with picric acid, in a Van Gieson type procedure, this dye demonstrates collagen and reticulin.

It is chemically very similar to Napthalene blue black CS, a dye recommended by Lendrum for the demonstration of the oldest fibrin but which is now difficult to obtain. Amido black 10B may be suitable as a substitute.


  1. R. D. Lillie.
    Conn’s Biological Stains
    Williams & Wilkins, Baltimore, MD., U.S.A.
  2. Aldrich chemical catalogue, 1992
    Aldrich Chemical Company, Milwaukee, WI, USA.
  3. Edward Gurr, 1971
    Synthetic dyes in biology, medicine and chemistry
    Academic Press, London, England.