Protocol
Pappenheim Stain
for Plasma Cells
Materials
Stock solution A
| Material | Amount | |
|---|---|---|
| Methyl green | 1 | g |
| Distilled water | 100 | mL |
| Phenol | 0.25 | g |
Stock solution B
| Material | Amount | |
|---|---|---|
| Pyronin Y | 4 | g |
| Distilled water | 100 | mL |
| Phenol | 5 | g |
Working solution
| Material | Amount | |
|---|---|---|
| Stock solution A | 15 | mL |
| Stock solution B | 35 | mL |
Tissue Sample
Most fixatives should be satisfactory.
Protocol
- Bring sections to water via xylene and ethanol.
- Place into the staining solution for 5-10 minutes.
- Rinse briefly with water.
- Dehydrate rapidly with acetone.
- Clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – violet
- Plasma cell cytoplasm – red
- Other cell cytoplasm – pink
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide. p. 355
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
